Phytochemical screening and Gas chromatography-mass spectrometry analysis on Ischaemumpilosum (Kleinex Willd.)

Abstract

Ischaemumpilosum (Kleinex Willd.) a weed among the grass is reported for ethno-medicinal practices for treatment of various treatments for human and domestic animals. The current work deals with phytochemical analysis in different parts of plants to find out bioactive compounds. The first-time reported results onI. pilosumreveal the significant phytochemicals by using preliminary phytochemical analysis, UV Visible spectral technique, FTIR analysis and GC-MS analysis. The preliminary phytochemical test confirms the presence of alkaloids, anthraquinone, cardiac glycosides, coumarins, flavonoids, glycosides, phenols, reducing sugars, saponins, steroids, tannin and triterpenes in Ischaemumpilosum.UV Visible spectra and FTIR gives the ranges of absorptions and functional group like Carboxylic acids (O-H) at 2956,92 cm-1, Alkanes (O-H) at 2849,89 cm-1, Aldehydes (C=O) at 1735,92 cm-1, Aromatic Rings (C=C) at 1462,95 cm-1, Alkanes (C-H) at 1377,97 cm-1, Esters (C-O) 1166,95 cm-1and Phenyl Ring (C-H) 758,97 cm-1. The GC-MS analysis related twenty-one compounds like Phenol, 4-bis (1,1-dimethylethyl), Pentanoic acid, 5-hydroxy, 2,4-di-t-butylphenyl esters, E-15-Heptadecenal, 1-Hexadecanol, n-Hexadecanoic acid, l (+)-Ascorbic acid 2,6-dihexadecanoate, Palmitic anhydride, Cycloeicosane, Cis-13-Octadecenoic acid and Triacontane from Ischaemumpilosumleaves extract.