Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract of Justicia procumbans leaf

Abstract

Objective: To perform Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract Justicia procumbans leaf. Methods: Proximate analysis in terms of ash value, extractive value was performed as per the standard method. Presence of secondary metabolites in the extract of Justicia procumbans was performed through various chemical tests. Folin-Ciocalteu assay method was used to determine total phenolic content and the in-vitro antioxidant activity was investigated in a dose-dependent manner with the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide free radical scavenging method. Results: Present studies revealed that the hydro-alcoholic extract of leaf contains secondary metabolites such as alkaloids, steroids, flavonoids, carbohydrates, proteins, and tannins. Total phenolic content was found to be 79.32±0.02 mg/gm by using the Linear Equation. IC50 value by DPPH and H2O2 methods was found to be 68.83µg/ml and 56.02 µg/ml respectively. Conclusion: It has been observed that the plant has high phenolic compounds and antioxidant activity. Therefore his plant can be the potent source of natural antioxidants as compared to synthetic compounds.