Phytochemical screening and evaluation of anti-inflammatory of activity of Gardenia carinata leaves

Abstract

Event Abstract Back to Event Phytochemical screening and evaluation of anti-inflammatory activity of Gardenia carinata leaves Velaga V. Appalaraju1*, Nahlah E. Ismail1, Senthil Adimoolam1 and Tan Xin Yi1 1 Faculty of Pharmacy, Mahsa University, Malaysia Background Gardenia carinata plant has wide range of therapeutic uses such as anti-HIV, cytotoxic, antimicrobial and mosquito larvicidal activities. The purpose of this study is to determine phytochemical screening and anti-inflammatory activity of different organic extracts of G. carinata leaves. Methods The leaf extracts were prepared using different organic solvents in the order of increasing polarity chloroform, methanol and distilled water by using soxhlet extraction method. The extract was characterized by Shinoda test, Salkowski test, ferric chloride test, Folin-Ciocalteau test and foam test. The anti-inflammatory activity of the entire G. carinata leaf extracts were evaluated at a dose level of 100 mg/kg (bw), orally by using Carrageenan-induced paw oedema method. Indomethacin 10 mg/kg (bw) was used as positive control. Paw thickness of rats were monitored every hour post oedema induction for 5 hours and the percentage of inhibited oedema were calculated. Results The inhibitory percentage exhibited by Gardenia carinata water extract (GCWE) was 32.66%, G. carinata methanol extract (GCME) was 27.98% and G. carinata chloroform extract (GCCE) 24.41% respectively. All these groups are significantly differenced in inhibition when compared with indomethacin inhibition percentage 39.33% at the fifth hour (p < 0.05). Extracts of G. carinata leaves revealed the presence of flavonoids, terpenoids, tannins, phenolic compounds and saponins. Conclusion Gardenia carinata leaves showed the anti-inflammatory activity in carrageenan-induced rat paw oedema model. Acknowledgements Authors thank MAHSA University for funding and providing the facilities to complete the research project. Keywords: Gardenia carinata, anti-inflammatory activity, Albino rats, Intraplantar administration, Carrageenan-induced paw oedema model Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Poster Presentation Topic: Inflammatory diseases Citation: Appalaraju VV, Ismail NE, Adimoolam S and Xin Yi T (2019). Phytochemical screening and evaluation of anti-inflammatory activity of Gardenia carinata leaves. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00097 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Velaga V Appalaraju, Faculty of Pharmacy, Mahsa University, Bandar Saujana Putra, Selangor, 42610, Malaysia, velagaappalaraju@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Velaga V Appalaraju Nahlah E Ismail Senthil Adimoolam Tan Xin Yi Google Velaga V Appalaraju Nahlah E Ismail Senthil Adimoolam Tan Xin Yi Google Scholar Velaga V Appalaraju Nahlah E Ismail Senthil Adimoolam Tan Xin Yi PubMed Velaga V Appalaraju Nahlah E Ismail Senthil Adimoolam Tan Xin Yi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.