Phytochemical screening and determination of treatment dose of Capparis spinosa L. roots extract in adjuvant induced arthritis in mice

Abstract

This study was aimed to investigate phytochemical properties of Capparis spinosa roots extract and to evaluate anti-arthritic activity of this extract in adjuvant-induced arthritis in mice which initiated using the complete Freund's adjuvant. For these purposes, the roots of C. spinosa were collected from different rural areas in Wasit province, and the extract was prepared and analyzed phytochemically. Also, a total of 42 male mice were prepared, divided equally to 7 groups involved 2 controls [negative (NC) and positive (PC)] as well as 5 treated (TG1, TG2, TG3, TG4 and TG5) received respectively 100, 150, 200, 250 and 300 mg/kg B.W of the extract for 21 days. Phytochemical analysis of C. spinosa L. roots extract showed a significant elevation in alkaloids and lacking of steroid, with existence of tannins, saponins, polysaccharides carbohydrates and glycosides, proteins and amino acid, coumarins, flavonoids, and polyphenolic compounds. The clinical findings of the right paw thickness for mice of different study groups were revealed a significant variation in their values. Comparing to NC, values of PC were increased significantly; while, the findings of treated groups with different concentrations of C. spinosa L. roots extract were variable significantly.