Phytochemical Screening and biological potentials of Musa × paradisiaca L. leaves extract as anticancer and antioxidant agents

Abstract

Background Many of these plants, have therapeutic effects and can be extracted and used in preparation of drugs, used directly or in combination with other plant extracts for medication which is a common practice in developing counties. Unfortunately, many of those who utilize these plants therapeutically do not have adequate knowledge or training in the safe use of the products. For these reasons, natural plant products need to be standardized and preliminary studies done to evaluate possible risks such as undesirable side effects, overdose and toxicity. Results Ethyl acetate extract of Musa × paradisiaca L shown anticervical carcinoma and anti-malignant melanoma activity in our study. Antioxidant activity demonstrated, that Musa × Paradisiaca L. leaves ethyl extract exhibited % inhibition at absorbance 517 nm with IC50 values = 3.70 to 45.50 at different concentration and compared with ascorbic acid as standard drug. Conclusions The present study indicates the anticancer and antioxidant activity on the basis of biological and phytochemical screening of Musa × paradisiaca L leaves extract. Ethyl acetate extract of leaves was evaluated for its anticancer activity. In vitro anticancer activity of extract were estimated by measuring significant inhibition of HeLa and A375 cell lines by MTT assay. The MTT assay clearly indicates that the inhibition or inhibitory activity of the extract was concentration dependent. Maximum inhibition of cell growth was found at the concentration of 320 µg/ml which was 54.35 and 55.97, respectively for HeLa and A375 cell lines. Therefore, 320 µg/ml concentration of extract was used to study the IC50 value that was calculated as 249.1 and 224.4, respectively. Antioxidant activity demonstrated that, plant extract exhibited percentage inhibition with IC50 values = 3.70 to 45.50 at different concentration and compared with ascorbic acid as standard drug.