Abstract
Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidant
Highlights
Free radicals are atoms or molecules that contain one or more unpaired electrons so that they become unstable and very reactive (Phaniendra & Babu, 2015)
The bad effects of free radicals can be overcome with reducing compounds known as antioxidants
April 2021, Volume 7, Issue 2, 275-280 was carried out using color testing of several secondary metabolites such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as testing the antioxidant activity of the ethanol extract of buni leaves using the DPPH method
Summary
Free radicals are atoms or molecules that contain one or more unpaired electrons so that they become unstable and very reactive (Phaniendra & Babu, 2015). According to Ramadenti, Sundaryono, & Handayani (2017), the presence of secondary metabolites such as alkaloids, saponins, tannins, flavonoids, steroids and terpenoids can act as antioxidants. Some of these secondary metabolites are found in the leaves of buni plants such as terpenoids (Elya et al, 2011) and flavonoids, saponins, tannins, steroids, and alkaloids (Islam et al, 2018) The existence of this content indicates that this plant has the potential to be efficacious as an antioxidant. April 2021, Volume 7, Issue 2, 275-280 was carried out using color testing of several secondary metabolites such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as testing the antioxidant activity of the ethanol extract of buni leaves using the DPPH method. The results of the antioxidant test will be expressed in the form of the IC50 value which is calculated based on the regression equation
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