Phytochemical profiling, antioxidant activity and antimicrobial activity of Argyrcia speciosa (Linn.f.) Sweet., collected from Darrang district, Assam

Abstract

The present study was carried out to analyze the phytochemical constituents, antioxidant activity, totalphenolic content, total flavonoid content, and antimicrobial activity of Argyrcia speciosa (Linn.f.) Sweet.Preliminary phytochemical analysis was done using standard procedure. Total phenolic content, totalflavonoid content, and antioxidant activities were determined spectrophotometrically in crude aqueous,methanol, chloroform, and hexane extracts. The in vitro antimicrobial activity was determined by well. diffusion method against seven MTCC bacterial species and two MTCC fungal species. Preliminaryphytochemical analysis indicated the presence of saponin, tannin, flavonoid, phenol, trapezoid, cardiacglycoside, and alkaloid, except anthraquinone, steroid, and reducing sugar. The chloroform extract showeda better result than the other extract. Clear antimicrobial activity was observed against Staphylococcusepidermidis and Escherichia coli using methanolic extract while aqueous extract showed moderate activity.Moderate activity was also noted against S. epidermidis and P. vulgaris using methanol and chloroformextract respectively. The percentages inhibition in the case of DPPH and ABTS were recorded highest as83.15 ± 0.000% and 94.80 ± 0.000 for chloroform and methanol extract respectively. The present studyrevealed that both primary and secondary metabolites are present in Argyrcia speciosa (Linn. f.) Sweet., which confer their traditional uses as food and medicine.