Phytochemical Profiling and GC-MS Analysis of Hybanthus species (Violaceae): Bioactive Properties

Abstract

In the field of pharmacology, medicinal plants are of great importance to researchers as most pharmaceutical industries depend on medicinal plant for their raw materials. Hybanthus enneaspermus and Hybanthus travancoricus belong to the family Violaceae and are well known for its medicinal properties. The present study was carried out to evaluate the possible bioactive components present in the above plant species. Petroleum ether, benzene, ethyl acetate, methanol, ethanol, and aqueous extracts were subjected to qualitative test for the identification of phytoconstituents as per standard procedure. The functional groups of plant powders were identified using FT-IR analysis. The ethanol extracts were analyzed via GC-MS techniques. The phytochemical screening of different solvent extract of H. enneaspermus and H. travancoricus revealed the presence of alkaloids, flavonoids, phenols, saponins, tannins, steroids, terpenoids, glycosides, carbohydrates and xanthoproteins. The FT-IR analysis confirmed the presence of hydroxyl, phenolics, alcohols, ester, amine, aromatic, alkanes and others. GC-MS analysis showed the presence of 15 for H. enneaspermus and 10 compounds for H. travancoricus, which include n-hexadecanoic acid, hexadecanoic acid, methyl ester, undecane, neophytadiene, etc. The phytochemical profile and GC-MS analysis of H. enneaspermus and H. travancoricus revealed the presence of important bioactive compounds with medicinal properties. Hence, the presence of these phytoconstituents is responsible for the therapeutic effects.