Phytochemical profile and in vitro evaluation of extracts from leaves of Drimys brasiliensis (Winteraceae) against bovine and equine herpesviruses

doi:10.7324/japs.2017.70719

Abstract

Highlights

Viral infections are a serious threat to human and animal health worldwide, and are of major economic importance both in developed and in developing countries, mainly owing to its high mortality and morbidity rates
The results obtained in this study indicate that the extracts from leaves of D. brasiliensis exhibited significant antiviral activity against animal herpesviruses, indicating their potential for medicinal use
Cytotoxicity assays The maximum non-toxic concentrations (MNTC) of the hydroalcoholic extracts from leaves of D. brasiliensis were evaluated in MDBK and Vero cells using the method described by Simoni et al (1996)

Summary

INTRODUCTION

Viral infections are a serious threat to human and animal health worldwide, and are of major economic importance both in developed and in developing countries, mainly owing to its high mortality and morbidity rates. Medicinal plants have various substances that inhibit different parts of the replication cycles of various types of DNA and RNA viruses. This antiviral activity is generally attributed to polyphenols, rosmarinic acid, and low- molecular- weight glycoside compounds such as chlorogenic acid and caffeic acid (Jassimand Naji, 2003). Medicinal plants are an important resource in the search for new ways to combat and control diseases caused by herpesviruses in both humans and animals (Khan et al, 2005). Essential oils from Drimys brasiliensis and D. angustifolia showed antiviral activity against HSV-1(Gomes, et al, 2013). Hydroalcoolic (EtOH:H2O 2:1) extracts from leaves of D. brasiliensis obtained from six different collections during one year (bimonthly during August/2008 to June/2009) were prepared and assayed against animal herpesviruses. The extract obtained from leaves at August/2008 was subjected to chromatographic separation procedures to afford three flavonoids – astilbin, isoastilbin and quercetrin, which were characterized by NMR spectral analysis

MATERIALSAND METHODS

Findings

CONCLUSIONS