Abstract
Winery industry by-products have a great reuse potential in the pharmaceutical and cosmetic fields due to their bioactive compounds. This study investigates the phytochemical profile and the bioactivity of Vitis vinifera variety Fetească neagră tendrils extract (TE) and leaves extract (LE), intended to be used in oral hygiene products recommended in periodontal disease. The evaluation of the phenolic content was performed by colorimetric analysis. Liquid chromatography coupled with mass spectrometry was used to determine the chemical profile of the two extracts obtained from V. vinifera. Moreover, the antioxidant activity of the extracts was determined by spectrophotometric methods, as well as on human gingival fibroblasts (HGF) cell line. The cytocompatibility and cytoprotective effect against nicotine-induced cytotoxicity were tested, as well as the anti-inflammatory and antimicrobial effects. The TE showed higher total polyphenolic content, rich in rutin, compared to the leaves extract that displayed important amounts of isoquercitrin. The antioxidant effect was confirmed by both non-cellular and cellular tests. The cytocompatibility of the extracts was confirmed at a wide range of concentrations. The cytoprotective effect was demonstrated in HGF exposed to cytotoxic doses of nicotine; 300 µg/mL of both tested extracts decreased nicotine toxicity by approximately 20%. When challenged with E. coli polysaccharides, in HGF cells co-exposed to TE and LE, a reduction in the release of proinflammatory cytokines (IL-8, IL-6 and IL-1β) was observed. The extracts were both able to reduce the levels of reactive oxygen species and inflammatory cytokines, and had notable antimicrobial effects on pathogenic bacteria associated with periodontitis.
Highlights
Vitis vinifera L. (V. vinifera) is a plant that belongs to Vitaceae family, which has been cultivated for more than 7500 years and is currently considered one of the most important fruit crops due to the great surfaces cultivated worldwide [1,2]
To the best of our knowledge, we report for the first time the cytoprotective, antioxidant, and anti-inflammatory properties of tendrils extract (TE) and leaves extract (LE), and based on our results, their possible use in the management and treatment of periodontal disease
absorbance of the samples (As) a result of the mentioned composition, both extracts displayed an antioxidant potential in the DPPH
Summary
Vitis vinifera L. (V. vinifera) is a plant that belongs to Vitaceae family, which has been cultivated for more than 7500 years and is currently considered one of the most important fruit crops due to the great surfaces cultivated worldwide [1,2]. Due to the rich content in bioactive compounds of winery by-products, the research conducted so far was focused on the mechanisms of actions of these phytochemicals and their possible use as food supplements; less attention was paid to their possible beneficial effects on oral health and disease prevention and progression at this level. In this context, the present study aimed to characterize the phytochemical composition of the less studied extracts of leaves and tendrils from V. vinifera subsp. The antimicrobial activity of these extracts was tested on several bacterial strains associated with the host inflammatory processes noted in periodontal disease
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
