Abstract
Background Sedum dendroideum has antioxidant effects that are beneficial for different diseases. We aimed to analyze the antiproliferative activity of S. dendroideum in human pterygium fibroblasts (HPFs). Methods HPFs were treated for 24 h with 0–1000 μg/mL of S. dendroideum lyophilized to analyze its effect on cell viability using the CellTiter assay. RNA from HPF treated with 250 μg/mL of S. dendroideum lyophilized was isolated, and the expression of VEGF and CTGF genes was evaluated by qPCR. A dermal fibroblast cell line (HDFa) was used as a healthy control. The total phenolic content, antioxidant activity, and chemical profile of S. dendroideum lyophilized were determined. Results Viability of HPF decreased after 24 h treatment of S. dendroideum in a dose-dependent manner. The expression of VEGF and CTGF significantly decreased (P < 0.01) in HPF treated with 250 μg/mL of S. dendroideum when compared with untreated HPF. The total phenolic concentration in the S. dendroideum lyophilized was 33.67 mg gallic acid equivalents (GAE)/g. Antioxidant activity was 384.49 mM Trolox equivalents/mL. The main phenolic compounds identified by HPLC analysis were the kaempferol-3-O-glycoside, kaempferol-3-O-rhamnoside, kaempferol-3-O-neohesperidoside-7-O-α-rhamnopyranoside, and kaempferol-3-O-glycoside-7-O-rhamnoside. Conclusions S. dendroideum decreases the proliferation of HPF and the expression of VEGF and CTGF. The phenolic compound concentration, antioxidant activity, and phytochemical profile may play a role in these effects.
Highlights
Succulent plants, or Sempervivum, are part of the Crassulaceae family and are well known for their medicinal properties. e Sedum genre has more than 30 species
Previous scientific reports proved its activity against pain in an induced peritonitis model, antioxidant activity used as a fresh leaf infusion, antiglycemic effect in diabetic mice, antiinflammatory activity in vitro due to its ability to stimulate macrophages to produce cytokines, and gastroprotective effect in vivo [1,2,3,4]. ese properties are related to vegetal secondary metabolites, including flavonoids glycosides, tannins, kaempferol, quercetin, and phenols
Given the molecular properties proven of S. dendroideum, we aimed to analyze its effect on human pterygium fibroblasts by means of proliferation along with the chemical profile of possible active compounds. us, this provides evidence for the development of a potential pharmacological treatment for pterygium
Summary
Sempervivum, are part of the Crassulaceae family and are well known for their medicinal properties. e Sedum genre has more than 30 species. Pterygium is a wing-shaped tissue growth over the conjunctiva It produces ocular irritation, foreign body sensation, and prurience, and its growth can interfere with the visual field [5]. We aimed to analyze the antiproliferative activity of S. dendroideum in human pterygium fibroblasts (HPFs). HPFs were treated for 24 h with 0–1000 μg/mL of S. dendroideum lyophilized to analyze its effect on cell viability using the CellTiter assay. RNA from HPF treated with 250 μg/mL of S. dendroideum lyophilized was isolated, and the expression of VEGF and CTGF genes was evaluated by qPCR. E total phenolic content, antioxidant activity, and chemical profile of S. dendroideum lyophilized were determined. S. dendroideum decreases the proliferation of HPF and the expression of VEGF and CTGF. E phenolic compound concentration, antioxidant activity, and phytochemical profile may play a role in these effects S. dendroideum decreases the proliferation of HPF and the expression of VEGF and CTGF. e phenolic compound concentration, antioxidant activity, and phytochemical profile may play a role in these effects
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