Abstract
Objective: The aim of this study is to determine the phytochemical contents and the antioxidant activity of Helianthemum lippii (L.) Dum.Cours. crude extract. Methods: For preliminary phytochemical analysis, standard procedures were applied, while identification and quantification of individual phenolic compounds were performed by HPLC analysis. The Folin–Ciocalteu method was used to evaluate the total phenolic acid content of the plant extracts, The total flavonoid content was determined using the aluminum chloride colorimetric assay. The FTIR spectroscopy method was used to examine the chemical makeup of the organic extracts. The antioxidant activities were assessed using the 1,1-diphenyl-2-picrylhydrazyl and reducing power assays. Results: Chemical analysis revealed the presence of numerous secondary metabolites, such as polyphenols, flavonoids, tannins, saponins, anthocyanins, cardiac glycosides, leuco anthocyanins steroids, terpenoids, alkaloids, and mucilage. For the HPLC analysis, we obtained 65 peaks and we identified 6 major elements of bioactive compounds. The total concentration of polyphenols and flavonoids was varied respectively 183.12±2.84 mg gallic acid eq/g dry wt and 72.00±1.03 mg quercetin eq/g dry wt /mg. The general concentration of condensed tannin and hydrolyzable tannin compounds were expressed in terms of catechin equivalent (5.88±1.58 mg Ca eq/g dry extract) and gallic acid (2.818±0.138 mgTA eq/g dry wt) respectively. FTIR spectroscopy investigation indicated several characteristic peak values in the extract with diverse functional groups such as amide, alcohol, and phenol groups. Concerning the antioxidant activity, we found that this extract has high inhibitory percentages equivalent to IC50 3.085±0.001 for DPPH and 1.724±0.021 for reduction power (µg/mL). Conclusion: Our study proved that the aqueous extract of the H lippii is very rich in secondary metabolites; in addition, it has a tremendous anti-oxidant capacity, which leads us forward to introduce it for medical use.
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