Abstract
Objectives: The present study investigates the protective effect of Kariyat against Indomethacin and Acetic Acid induced Inflammatory Bowel Disease in male wistar rats. Material Method: HPTLC and GC-MS investigations indicated presence of steroid, phenols, terpenoid, alkaloids, saponine, flavonoids. IBD was induced by administration of Indomethacin (7.5mg/kg b.w. S.c.), Acetic Acid (4% v/v). Two different models used to induce IBD named Indomethacin induces enter colitis and Acetic Acid induces enter colitis in case of Indomethacin induces enter colitis the compromises 5 groups (n=6), normal, control, standard treated (Prednisolone 2mg/kg p.o.), KEE treated (100 mg/kg b.w.), KEE treated (200 mg/kg b.w.) in case of Acetic Acid induces colitis the compromises 5 groups (n=6), normal, control, standard treated (Prednisolone 2mg/kg p.o.), KEE treated (100mg/kg b.w.), KEE treated(200mg/kg b.w.). After treatment of 7 days animals were sacrificed and colon was isolated for macroscopic and microscopic studies. Quantification of inflammation was done by using myeloperoxidase assay (MPO), Lactate dehydrogenase (LDH), Lipid peroxidase (LPO). Result: Evaluation based on macroscopic features showed significantly lower score values for drug treated and standard drug treated groups compared to the disease control groups. Histological examination of disease control group showed massive necrosis of the mucosa and sub mucosa. Drug treated group showed mild lesions, regeneration and inflammatory reaction. The Prednisolone treated group showed suppressed inflammatory reaction. The results observed from MPO, LDH and LPO assays showed significant improvement of disease with extract treated groups compared to disease control group. Histopathological examination of Kariyat treated group revealed less damage compared to Indomethacin and Acetic Acid Induced group. Conclusion: Kariyat have shown to be effective in Indomethacin and Acetic Acid induced colitis in rats, which has protected the animals against experimentally induced disease because of its antioxidant and anti-inflammatory activity.
Highlights
Inflammatory bowel disease (IBD) is a spectrum of chronic idiopathic inflammatory intestinal conditions
Kariyat Ethanolic Extract (KEE) showed the presence of andrographolide and which was confirmed with retardation factor Rf 0.6
The KEE showed the presence of andrographolide in the respective Rf region, which matched very well with that of standard andrographolide (Figure 1)
Summary
Inflammatory bowel disease (IBD) is a spectrum of chronic idiopathic inflammatory intestinal conditions. IBD conventionally is divided into two major subtypes: Ulcerative colitis and Crohn’s disease. Ulcerative colitis is characterized by confluent mucosal inflammation of the colon starting at the anal verge and extending proximally for a variable extent (e.g., proctitis, left-sided colitis, or pan colitis). Crohn’s disease, by contrast, is characterized by trans mural inflammation of any part of the gastrointestinal tract but most commonly the area adjacent to the ileocecal valve.[1] Both diseases increase the risk of adenocarcinoma of the colon in the affected area.[2] Ulcerative colitis is most prevalent in North America, North Europe and Australia. The prevalence is roughly 10 times lower in southern and Eastern Europe, Africa, Asia and South America. Some reports have indicated that disease is seen with increased frequency in parts of Asia such as India, Bangladesh and Japan.[3]
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