Abstract
The present study was aimed to analysis of bioactive constituents of Papaver somniferum (Poppy seed). The ethanol extract of the seeds were subjected to Phytochemical Screening, Gas chromatography- mass spectroscopic (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis. GC-MS analysis of the seeds was performed using a Scion 436- GC Bruker model nd Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) and IR spectrum was recorded in spectrophotometer (Shimadzu, IR Affinity1, Japan). Phytochemical screening for seeds extracts indicated the presence of various secondary metabolites like Alkaloid, Cardiac Glycosides, Flavonoid, Phytosterols and Terpenoids. GC-MS analysis of compounds with totally, Thirty Nine volatile compounds major chemical compounds were identified, such as 9-Octadecynoic acid(30.72%), 9-Tetradecen-1-ol, acetate, (E)- (24.02%), 9,12-Octadecadienoic acid, methylester, (E,E)- (7.82%), cis-9,10-Epoxyoctadecan-1-ol (7.43%) and Undec-10-ynoic acid(4.36%). FT-IR analysis of peak values with various functional compounds such as alcohols, phenols, carboxylic acids, aldehydes, amides, amino acids, anhydrides, esters, ketones, Unsaturated aliphatics, aromatics, unsaturated heterocycles, amines, Nitro compound, Alkanes, alkenes, sugars,Sulphur, phosphorus, and fluorine compounds. The present results concluded that the phytochemicals was observed in ethanol extract which revealed that the Papaver somniferum (Poppy seed) is potential use in different fields namely medical and pharmaceuticals and greatly valuable in medicinal practice for the treatment of several human aliments. Keywords: GC-MS, FT-IR, Papaver somniferum L and NIST.
Highlights
Cardiac GlycosidesGC MS Analysis The compounds present in the ethanolic extract of Papaver somniferum L, were identified by Gas chromatography- mass spectroscopic (GC-MS) analysis (Fig. 1)
Spices have been defined as plant substances from on bread or rolls. (Erinç et al,. 2009)
The Papaver somniferum L Spice in ethanol extract with the Papaver somniferum L. belongs to the Papaveraceae aid of Gas chromatography- mass spectroscopic (GC-MS) and FT-IR techniques, which may offer a family, and is commonly known as “Opium poppy.”
Summary
GC MS Analysis The compounds present in the ethanolic extract of Papaver somniferum L, were identified by GC-MS analysis (Fig. 1). Thirty Nine volatile compounds from ethanolic extract of Papaver somniferum L were separated and identified by GCMS. The GC-MS analyses of Papaver somniferum L established the identification of 39 volatile compounds in the ethanolic extract. The composition are as follows: 9Octadecynoic acid (30.72%), 9-Tetradecen-1-ol, acetate, (E)- (24.02%), 9,12-Octadecadienoic acid, methyl ester,. The chemical group classifications are as follows: Monoterpenes (1.33%), Aromatic (0.47%), Amino acid (1.42), Fatty acid (51.03%), Acetate (24.31%), Nitrogen compounds (0.14%), Alcohol (0.73%), Aldehyde (0.33%), Alkanes (1.22%), Alkenes (1.07), Esters (0.94%), Epoxy compounds (2.23%), naphthalene (0.71%) and ketones (0.75%)
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