Phytochemical estimation and evaluation of antioxidant activity of hydro-alcoholic and ethylacetate extracts of Tribulus terrestris linn. (gokhru) and Solanum nigrum linn (makoi)

Abstract

Tribulus terrestris L. and Solanum nigrum L. has been used traditionally since ages. To explore the phytoconstituents (qualitatively and quantitatively) and estimate antioxidant activity of hydroalcoholic and ethyl acetate extracts of fruits of Tribulus terrestris L. and Solanum nigrum. Different methods were adopted for phytochemical screening. Evaluation of antioxidant potential was achieved by total anti-oxidant capacity assay, hydrogen peroxide scavenging activity, DPPH radical-scavenging activity, Nitric oxide radical scavenging activity and reducing power assay. The qualitative estimation of fruits of Tribulus terrestris L and of Solanum nigrum L. showed the presence of various phytoconstituents likewise tannins, saponins, flavonoids and terpenoids. Quantitative estimation revealed the presence of flavonoids (5.01%w/w), saponins (0.70%w/w), and terpenoids (0.013%w/w) in fruits of Tribulus terrestris L. whereas presence of flavonoids (10.46%w/w), saponins (11.02%w/w) and terpenoids (0.034%w/w) was found in fruits of Solanum nigrum L. It can be concluded that both the extracts of Tribulus terrestris L and Solanum nigrum L possess significant antioxidant activity.