Phytochemical Contents and in Vitro Antioxidant Activities of Aqueous, Hydroalcoholic and Methanolic Extracts of Shankhapushpi (Clitoria Ternatea. L) Plant Cultivated under Polyhouse and Open Field Conditions

Abstract

ABSTRACT Shankhapushpi (Clitoria ternatea) grown under polyhouse and open-field conditions were investigated for physicochemical parameters of their powdered samples, qualitative and quantitative analysis of phytochemicals, antioxidant capacities and development of chemical profiles using solvent extracts in methanol, hydroalcoholic and water. The two samples were similar in their physicochemical parameters except for the higher extractive yield from the open-field grown plants. Although the primary metabolites were equal in the polyhouse and field-grown, the secondary metabolites were higher in the polyhouse-grown plant samples. Methanolic extract of polyhouse-grown samples was richer in phenolics, flavonoids, tannins, and triterpenoids than the open-field-grown ones. The DPPH radical and hydroxyl radical scavenging activity, ferric reducing activity, and total antioxidant activity, values were higher in methanolic extract of polyhouse-grown plant sample. There was a positive correlation between phytochemicals and antioxidant capacities: in the polyhouse-grown samples the total phenol content correlated with DPPH assay, while in the open-field-grown plant samples it correlated with hydroxyl radical assay.