Abstract
BackgroundThere is a need for the screening more effective, affordable and readily available antimicrobial substances from local medicinal plants or herbs as the pathogenic bacteria are developing the resistance to common antibiotics. Guava (Psidium guajava L.) has antimicrobial activities and it is easily accessible to local populace in tropical region. Antimicrobial properties of guava extracts are attributed to the presence of different phytochemical constituents. The fact that phytochemicals’ constituents vary as a result of geographical locations and literatures about Ethiopian guava with respect to phytochemicals’ composition and content, and antimicrobial activities are hardly available, there is a need to analyze phytochemicals and antimicrobial properties of the Ethiopian guava from Oromia Regional State, Babile and Gursum Districts against Salmonella Typhi, Shigella boydii, Staphylococcus aureus and Enterococcus faecalis.MethodsExtraction was done by maceration. Qualitative analysis of phytochemicals was carried out using standard protocol and quantitative phytochemical determination was conducted using spectrophotometric and gravimetric methods. Antimicrobial activities were determined by disc diffusion and broth dilution method.ResultsQualitative phytochemical analysis revealed the presence of alkaloids, saponin, steroids and tannin, but the absence of flavonoid and phlobatannin in all Babile and Gursum leaf and bark extracts. Gravimetric measurement showed the highest terpenoid content of 105.00 ± 8.66 mg extract/g of powder in Gursum leaf extract. Similarly, spectrophotometric measurement showed the highest total phenol content of 0.205 ± 0.01 mg/g of the extracts as Tannic Acid Equivalent in Gursum leaf extract. Antimicrobial activity tests revealed that Babile leaf extract showed the highest 13.0 ± 6.79 mm zone of inhibition against Shigella boydii compared to that of other guava extracts. Babile leaf extract showed the lowest 2.375 ± 0.177 mg/ml minimum inhibitory concentration against Shigella boydii, while Gursum leaf extract showed the lowest 1.875 ± 0.884 mg/ml MIC against Salmonella Typhi.Phytochemical analysis showed the presence of alkaloids, saponins, steroids and tannins in the all extracts, but the absence of flavonoids and phlobatannins in the all extracts and terpenoids in Babile extracts. The highest content of alkaloid and terpenoid (98.67 ± 14.43, 93.33 ± 8.82 mg extract/g of powder, respectively) were found in Babile leaf and Gursum bark extracts, respectively. Antimicrobial activity tests revealed that Babile leaf extracts showed higher zone of inhibition against all clinical isolates than that of Gursum leaf extracts, but Babile bark extracts showed lower zone of inhibition against all clinical isolates than that of Gursum bark extracts. Babile leaf extracts showed the highest zone of inhibition (13.0 ± 6.79 mm) against S. boydii, and Babile bark extracts showed the lowest Minimum Inhibitory Concentration (1.250 ± 0.001 mg/ml) against S. Typhi.ConclusionGuava extracts from different location could be source of natural antimicrobial agents with different composition and content. In vivo antimicrobial activity, and isolation, identification and synergy of specific active compound that responsible for the antibacterial activity should be evaluated.
Highlights
There is a need for the screening more effective, affordable and readily available antimicrobial substances from local medicinal plants or herbs as the pathogenic bacteria are developing the resistance to common antibiotics
Antimicrobial activity of extract of leaf was carried out using well diffusion method and the results showed 3, 7, 6, 5 and 4 mm Zone of inhibition (ZOI) against S. typhi, Salmonella paratyphi A, Salmonella paratyphi B, Shigella sonnei, Shigella dysentarie, respectively [2]
Phytochemical analysis revealed that Babile and Gursum guava leaf and bark extracts showed slight variation in composition and content of phytochemicals’ constituents
Summary
There is a need for the screening more effective, affordable and readily available antimicrobial substances from local medicinal plants or herbs as the pathogenic bacteria are developing the resistance to common antibiotics. Plants produce a diverse array of secondary metabolites, many of which have antimicrobial activities against some pathogenic microorganisms that are implicated in enteric infections. Some of these compounds are constitutive, existing in healthy plants in their biologically active forms and they elicit chemotherapeutic or chemoprophylactic properties against a wide range of infectious enteric diseases [1]. Potential effort has been the screening of more effective, affordable and readily available antimicrobial substances with diverse chemical structures and novel mechanisms of action from local medicinal plants or herbs [4, 5]. All parts of the plant have been used for different purposes: hepatoprotection, antioxidant, antiinflammatory, anti-spasmodic, anti-cancer, anti-microbial, anti-hyperglycemic, analgesic, endothelial progenitor cells, anti-stomachache and anti-diarrhea [7]
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