Abstract
Potentilla anserina L. (Rosaceae) is known for its beneficial effects of prevention of pre-menstrual syndrome (PMS). For this reason P. anserina is processed into many food supplements and pharmaceutical preparations. Here we analyzed hydroalcoholic reference extracts and compared them with various extracts of different pharmacies using an integrative metabolomics platform comprising GC-MS and LC-MS analysis and software toolboxes for data alignment (MetMAX Beta 1.0) and multivariate statistical analysis (COVAIN 1.0). Multivariate statistics of the integrated GC-MS and LC-MS data showed strong differences between the different plant extract formulations. Different groups of compounds such as chlorogenic acid, kaempferol 3-O-rutinoside, acacetin 7-O-rutinoside, and genistein were reported for the first time in this species. The typical fragmentation pathway of the isoflavone genistein confirmed the identification of this active compound that was present with different abundances in all the extracts analyzed. As a result we have revealed that different extraction procedures from different vendors produce different chemical compositions, e.g. different genistein concentrations. Consequently, the treatment may have different effects. The integrative metabolomics platform provides the highest resolution of the phytochemical composition and a mean to define subtle differences in plant extract formulations.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-012-0473-x) contains supplementary material, which is available to authorized users.
Highlights
Potentilla anserina L. belongs to the family of Rosaceae and its extracts have been used for a long time in traditional medicine
Tomczyk and Lattereport that P. anserina and other Potentilla species are generally used to prepare homeopathic medications (Tomczyk and Latte 2009) according to homeopathic pharmacopoeias like Homeopathic Pharmacopoeia of the United States (HPUS) and German Homeopathic Pharmacopoeia (HAB) (Hiller 1994). For this reason P. anserina is processed into many food supplements and pharmaceutical preparations such as teas, tinctures, capsules, tablets, and juice and is consumed by women in order to prevent the symptoms of pre-menstrual syndrome (PMS)
The aim of the present work is to characterize the phytochemical profile of hydroalcoholic extracts of P. anserina and its commercial products prepared by different pharmacies using a comprehensive metabolomics platform integrating Gas chromatography coupled to mass spectrometry (GC-MS), LC-MS and multivariate statistics (Fig. 1)
Summary
Potentilla anserina L. (silverweed) belongs to the family of Rosaceae and its extracts have been used for a long time in traditional medicine. Tomczyk and Lattereport that P. anserina (aerial parts or the whole plant) and other Potentilla species are generally used to prepare homeopathic medications (Tomczyk and Latte 2009) according to homeopathic pharmacopoeias like Homeopathic Pharmacopoeia of the United States (HPUS) and German Homeopathic Pharmacopoeia (HAB) (Hiller 1994). For this reason P. anserina is processed into many food supplements and pharmaceutical preparations such as teas, tinctures, capsules, tablets, and juice and is consumed by women in order to prevent the symptoms of pre-menstrual syndrome (PMS). The aim of the present work is to characterize the phytochemical profile of hydroalcoholic extracts of P. anserina and its commercial products prepared by different pharmacies using a comprehensive metabolomics platform integrating GC-MS, LC-MS and multivariate statistics (Fig. 1)
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