Abstract
Purpose : To investigate the phytochemical profile and antihyperplasic effect of ethanol extract of Fructus hordei. G (FHE) on hyperplasia of mammary gland (HMG) in rats. Methods : The Fructus hordei. G extract (FHE) was obtained by a rotary vacuum evaporator. The total flavonoids, total alkaloids and total phenolic compounds in FHE were assayed by aluminum chloride colorimetric method, acid-dye colorimetry and Folin-Ciocalteu method, respectively. HMG model rats were prepared for the experiments by injecting estradiol and progesterone. After oral administration with 150, 300 and 600 mg/kg of FHE for 30 days, breast nipple height, and serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), 5-hydroxytryptamine (5-HT), estradiol (E2), progesterone (P), prolactin (PRL), follicle stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropin-releasing hormone (GnRH) levels of the rats were measured by ELISA kits. The pathological morphology of mammary gland tissues was examined by optical microscopy. Results : The ethanol extract of FHE contained 1.88 ± 0.05 mg total flavonoids/g as quercetin equivalent (QE), 21.93 ± 0.06 mg hordenine equivalent (HE) of total alkaloids/g extract and 70.64 ± 0.11 mg total phenols/g gallic acid equivalent (GAE). The extract significantly diminished breast nipple height, MDA, 5-HT, E2, PRL, FSH and GnRH levels, and increased SOD, GSH-Px, P and LH levels (p < 0.05). FHE inhibited the alveolus, lobules and secretion of the mammary gland. Conclusion : Fructus hordei extract has significant antihyperplasia effect on mice mammary gland by regulating serum sex hormones. Keywords : Fructus Hordei, Flavonoids, Alkaloids, Phenolics, Hyperplasia Mammary gland
Highlights
Hyperplasia of mammary gland (HMG) is a kind of pathological hyperplasia of the lobules of the mammary gland induced by balance disorder of estrogen and progesterone [1]
We explored the effects of Fructus hordei G. extract (FHE) on HMG in rats
When extraneous stimulations act on human body, some sex hormones secrete abnormally leading to many kinds of disease such as HMG, hyperprolactinemia, hysteromyoma and infertility
Summary
Hyperplasia of mammary gland (HMG) is a kind of pathological hyperplasia of the lobules of the mammary gland induced by balance disorder of estrogen and progesterone [1]. We explored the effects of Fructus hordei G. extract (FHE) on HMG in rats. The content of total phenolic compounds was expressed as mg gallic acid equivalent/g of dry extract.
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