Phytochemical Characterization, In Vitro Anti-Inflammatory, Anti-Diabetic, and Cytotoxic Activities of the Edible Aromatic Plant; Pulicaria jaubertii.

Hamdoon A Mohammed,Ehab A Ragab,Mohammed F Abdelwahab,El-Sayed M El-Ghaly

doi:10.3390/molecules26010203

Hamdoon A Mohammed, Ehab A Ragab + Show 2 more

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https://doi.org/10.3390/molecules26010203

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Abstract

Pulicaria jaubertii is a medicinal herb that alleviates inflammations and fever. Chromatographic separation, phytochemical characterization, and in vitro biological activities of the plant n-hexane extract were conducted for the first time in this study. Six compounds were isolated for the first time from the n-hexane fraction of Pulicaria jaubertii aerial parts and were identified on the bases of NMR and MS analyses as pseudo-taraxaterol (1), pseudo-taraxasterol acetate (2), 3β-acetoxytaraxaster-20-en-30-aldehyde (3), calenduladiol-3-O-palmitate (4), stigmasterol (5), and α-tocospiro B (6). Compound (6) was a rare tocopherol-related compound and was isolated for the first time from family Asteraceae, while compound (3) was isolated for the first time from genus Pulicaria. The total alcoholic extract and n-hexane fraction were tested for their anti-inflammatory, antidiabetic, and cytotoxic activities. The n-hexane fraction has dose dependent red blood cells (RBCs) membrane stabilization and inhibition of histamine release activities with IC50: 60.8 and 72.9 µg/mL, respectively. As antidiabetic activity, the alcoholic extract exerted the most inhibition on the activity of yeast α-glucosidase, with an IC50: 76.8 µg/mL. The n-hexane fraction showed cytotoxic activity against hepatocarcinoma (HepG-2), breast carcinoma (MCF-7), and prostate carcinoma (PC-3) cell lines with IC50: 51.8, 90.8 and 62.2 µg/mL, respectively. In conclusion, the anti-inflammatory effect of Pulicaria jaubertii might be attributed to the triterpenoid constituents of the n-hexane extract of the plant.

Highlights

Medicinal plants and their products have been used in human life quality improvement [1]
Genus Pulicaria, belonging to the Asteraceae family, tribe Inuleae, consists of 100 species distributed in Europe, North Africa, and Asia, especially in Yemen and Saudi Arabia and is known for its medicinal properties [4]
The total alcoholic extract of Pulicaria jaubertii was fractionated between non the n-hexane soluble using different techniques, i.e.,chromatographic

Summary

Introduction

Medicinal plants and their products have been used in human life quality improvement [1]. They have been used in disease remediation processes around the world and their natural derived product is still the focus of researchers in the drug discovery and in the areas of biology [1,2,3]. Genus Pulicaria, belonging to the Asteraceae family, tribe Inuleae, consists of 100 species distributed in Europe, North Africa, and Asia, especially in Yemen and Saudi Arabia and is known for its medicinal properties [4]. In Yemen and Saudi Arabia, plants of genus Pulicaria are known for their common uses as herbal teas, insect repellant, and alleviate inflammations [4].

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