Phytochemical Characterization & investigation of Haldina cordifolia Leaf extract as potential herbal drug

Abstract

The current learning was intended to analyze about the important phytoconstituents of methanolic fraction of Haldina cordifolia leaves extract. Qualitative extraction of Haldina leaves was prepared using Methanolic extract. H cordifolia leave extract confirms presence of Flavanoids, Alkaloids, phenols, saponins, anthraquinone, Phytosterols, coumarins and anthocyanins, which was confirmed from the TLC analysis. The FT-IR spectrum confirmed presence of alkyl, methyl, ether, amino, alkene, alcohol, carboxyl and carbonyl groups. UV spectral reports indicate presence of unsaturated groups and hetero atoms such as S, N, and O [8]. The absorption spectrum for H. cordifolia extract shows five identifying peaks and confirms presence of organic chromophores and variety of functional groups within the H. cordifolia extract. LC-MS analysis data suggests existence of hydroxyl coumarins compounds namely scopoletin , isoscopoletin , umbelliferone and phytosterols like ß- sitosterol,epiglobulol, campesterol and Caryophylene oxide etc., that can be considered for antimicrobial activity and it might largely contribute in action againest multidrug resistant microbes. Keywords: IR-spectroscopy, LC-MS, Scopoletin, Umbelliferone, ß-sitosterol and Campesterol.