Abstract
The current learning was intended to analyze about the important phytoconstituents of methanolic fraction of Haldina cordifolia leaves extract. Qualitative extraction of Haldina leaves was prepared using Methanolic extract. H cordifolia leave extract confirms presence of Flavanoids, Alkaloids, phenols, saponins, anthraquinone, Phytosterols, coumarins and anthocyanins, which was confirmed from the TLC analysis. The FT-IR spectrum confirmed presence of alkyl, methyl, ether, amino, alkene, alcohol, carboxyl and carbonyl groups. UV spectral reports indicate presence of unsaturated groups and hetero atoms such as S, N, and O [8]. The absorption spectrum for H. cordifolia extract shows five identifying peaks and confirms presence of organic chromophores and variety of functional groups within the H. cordifolia extract. LC-MS analysis data suggests existence of hydroxyl coumarins compounds namely scopoletin , isoscopoletin , umbelliferone and phytosterols like ß- sitosterol,epiglobulol, campesterol and Caryophylene oxide etc., that can be considered for antimicrobial activity and it might largely contribute in action againest multidrug resistant microbes. Keywords: IR-spectroscopy, LC-MS, Scopoletin, Umbelliferone, ß-sitosterol and Campesterol.
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