Abstract
The phytochemical, antibacterial, antifungal and MIC (minimum inhibitory concentration) study of the leaves and bark of Colebrookea oppositifolia Smith was evaluated. Phytochemical screening C. oppositifolia Sm. indicated the presence of alkaloids, flavonoids, glycosides, steroids and saponins whereas terpenoids, tannins, and cardiac glycosides are showed only in bark extract. Petroleum ether extract showed maximum effectiveness against P. aeruginosa with bark extract (18±2.2 mm) and maximum antifungal potential exhibited by petroleum ether extract of leaves (15 ±0.3 mm) against A. nigar. It was noticed that the extracts in distilled water had not shown much potential. The MIC assay was carried out for further verification of results obtained by the Zone of Inhibition against both bacterial and fungal strains. The lowest MIC value is 0.09±0.3 at 1 mg/mL against E. coli exhibited by bark extract and 0.09±0.2 at 0.9 mg/mL against K. pneumoniae shown by leaf extract. Whereas, in fungi minimal MIC value exhibited 0.11±0.3 at 0.9mg/mL by leaf extract against A. oryzae. Hence, it is recommended further investigation to explore toxicological and clinical trials before using this plant as drug. Keywords: Antibacterial; Antifungal;Colebrookea oppositifolia; Ethnomedicinal plant http://dx.doi.org/10.19045/bspab.2018.70017
Highlights
) study of the leaves and bark of Colebrookea oppositifolia Smith was evaluated
minimum inhibitory concentration (MIC) value is 0.09±0.3 at 1 mg/mL against E. coli exhibited by bark extract and 0.09±0.2 at 0.9 mg/mL against K. pneumoniae shown by leaf extract
To checked the antimicrobial activity of the plant material i.e. Colebrookea oppositifolia Smith, extracts were prepared. Macerated and their % yield was intended before further analysis, to provide glance of the economical perspective of the completepractice.The phytochemical screening the plant extracts of C
Summary
) study of the leaves and bark of Colebrookea oppositifolia Smith was evaluated. Phytochemical screening C. oppositifolia Sm. indicated the presence of alkaloids, flavonoids, glycosides, steroids and saponins whereas terpenoids, tannins, and cardiac glycosides are showed only in bark extract. MIC value is 0.09±0.3 at 1 mg/mL against E. coli exhibited by bark extract and 0.09±0.2 at 0.9 mg/mL against K. pneumoniae shown by leaf extract. Six bacterial strains including three gram-positive (Staphylococcus aureus, Bacillus subtilis, Enterococcus facealis) and three gram negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) were used for the estimation of antimicrobial activity of bark and fruit of Cholebrookia oppositifolia Smith. The aqueous extracts of bothparts of C. oppositifolia Smith had showed satisfactory results against the particular bacterial strains (Figure 4).
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