Abstract
BackgroundThe current study was conducted to evaluate the antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activities of ethanolic extract of Lepisanthes rubiginosa L. leaves in different experimental models.MethodsQuantitative and qualitative analysis were done by TLC (thin layer chromatography) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging assay. Analgesic, antihyperglycemic and antidiarrheal activities were evaluated using acetic acid induced writhing in mice, oral glucose tolerance test and castor oil induced diarrhea, respectively. Neuropharmacological activity was investigated in mice using both Open Field and Hole Board methods.ResultsTLC analysis indicated the presence of antioxidant compounds in the extract we used. The extract showed IC50 value was 31.62 μg/mL whereas the standard ascorbic acid showed 12.02 μg/mL. In acetic acid induced writhing assay, the extract showed 46.07% and 58.43% writhing inhibition at the doses of 250 mg/kg and 500 mg/kg body weight, respectively whereas standard diclofenac-Na (25 mg/kg) showed 86.52% writhing inhibition. The plant extract showed significant (p < 0.05) antihyperglycemic activity on mice as compared to control groups. In neuropharmacological activity assay the experimental animal showed a noticeable decrease in locomotion by showing a decrease in number of square crossed and head dipping at both doses (250 mg/kg & 500 mg/kg). In antidiarrheal activity test, the plant extract at the doses of 250 mg/kg and 500 mg/kg showed percent inhibition of defecation 57.89 and 77.19 respectively, whereas standard loperamide (3 mg/kg) showed percent inhibition of defecation 88.59.ConclusionThe results demonstrated that the extract has potential antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activity.
Highlights
The current study was conducted to evaluate the antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activities of ethanolic extract of Lepisanthes rubiginosa L. leaves in different experimental models
It is assumed that the leaf extract should exert neuropharmacological activity, and we carried out in-vivo experiments to evaluate neuropharmacological activity
Phytochemical screening Phytochemical studies showed that carbohydrate, alkaloid, glycoside, phenolic compounds, flavonoids, tannins, steroids and proteins were present whereas combined reducing sugar and gums were absent in the ethanol extract of L. rubiginosa (Table 1)
Summary
The current study was conducted to evaluate the antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activities of ethanolic extract of Lepisanthes rubiginosa L. leaves in different experimental models. Medicinal plants provide an excellent source of lead compound in discovering noble and new drugs with considerable less side or adverse effects [1]. Hasan et al BMC Complementary and Alternative Medicine (2017) 17:496. This has resulted in an enormous increase of research on different medicinal plant to find lead compounds responsible for such pharmacological activity. It is seen that an aqueous fruit extract of L. rubiginosa at doses of 20 mg/kg and 100 mg/kg significantly (p < 0.05) decrease locomotion and at dose of 100 mg/kg enhanced the thiopentalinduced sleeping period. It is assumed that the leaf extract should exert neuropharmacological activity, and we carried out in-vivo experiments to evaluate neuropharmacological activity
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