Abstract
Pharmacognosy Research,2021,13,4,208-217.DOI:10.5530/pres.13.4.13Published:October 2021Type:Original ArticleAuthors:Vinod Gahlot, and Dinesh Kumar Yadav Author(s) affiliations:Vinod Gahlot1, Dinesh Kumar Yadav2,* 1Department of Pharmacology, SGT College of Pharmacy, SGT University, Gurugram, Haryana, INDIA. 2Department of Pharmacognosy, SGT College of Pharmacy, SGT University, Gurugram, Haryana, INDIA. Abstract:Background: Quality-based assessment of herbal drugs or products is being more concerning for their quality, safety and regulatory purpose. A. mexicana is a traditional herbal medicine that has a long history in the treatment of arthritis, anti-fungal anti-cancer and brain disorders. Aim: Due to lack of scientific evidence based on phytopharmacology, the study is aimed for phytochemical and antiepileptic evaluation of identified metabolites in A. mexicana. Materials and Methods: Phytochemical identification was done using MS, FTIR and 1H-NMR and quantitated using HPTLC densitometric analysis. Further, ADME and network pharmacology studies were performed to evaluate biological response of identified metabolites. Results: The resulted outcomes of the spectral analysis suggest isolated compounds as ferulic acid, caffeic acid, berberine and angoline. In HPTLC quantitative analysis, the content of ferulic acid, caffeic acid, berberine and angoline was found as 3.475 ± 0.028, 1.036 ± 0.013, 0.714 ± 0.014 and 0.738 ± 0.081 μg/mg of A. mexicana extract. In ADME analysis, berberine and angoline showed good bioavailable response while in network pharmacology analysis, except angoline, all the metabolites significantly interacted with several genes (SOD1, NOS, MAPK3, UGT1As, G6PD, ACOT2, BAAT etc) associated with brain ischemia, oxidative and inflammatory stress or the genes response for elimination of toxins from the body. Conclusion: Hence, the study enlightens that A. mexicana possess several major and minor metabolites and which can be the key parameter for further quality and regulatory based assessment of A. Mexicana and biological role against oxidative and inflammatory stress induced epileptic seizure. Keywords:ADME analysis, Argemone mexicana L., HPTLC, Network pharmacology, PhytoconstituentsView:PDF (1.81 MB)
Highlights
The application for authentication and reliable quality studies are the principal requirement for herbal raw materials or medicinal plants used under the traditional system of medicine including the Indian system of medicine, the Chinese system of medicine etc.[1,2] Historically, medicinal plants have been used in the treatment of various disorders from a long time while many of the traditional scripture classify them as per their applicability, accessibility and admissibility to cure, treat and nullify the disease or any deleterious effects arise in the body’s system.[3]
In ADME analysis, berberine and angoline showed good bioavailable response while in network pharmacology analysis, except angoline, all the metabolites significantly interacted with several genes (SOD1, NOS, MAPK3, UGT1As, Glucose-6-Phosphate Dehydrogenase (G6PD), ACOT2, BAAT etc) associated with brain ischemia, oxidative and inflammatory stress or the genes response for elimination of toxins from the body
The analysis showed ferulic acid with significantly interaction with the genes such as MAPK1, MAPK3, UGT1As, G6PD, ACOT2 BAAT etc which regulates inflammation.[32]
Summary
The application for authentication and reliable quality studies are the principal requirement for herbal raw materials or medicinal plants used under the traditional system of medicine including the Indian system of medicine, the Chinese system of medicine etc.[1,2] Historically, medicinal plants have been used in the treatment of various disorders from a long time while many of the traditional scripture classify them as per their applicability, accessibility and admissibility to cure, treat and nullify the disease or any deleterious effects arise in the body’s system.[3]. ADME and network pharmacology studies were performed to evaluate biological response of identified metabolites. In HPTLC quantitative analysis, the content of ferulic acid, caffeic acid, berberine and angoline was found as 3.475 ± 0.028, 1.036 ± 0.013, 0.714 ± 0.014 and 0.738 ± 0.081 μg/mg of A. mexicana extract. In ADME analysis, berberine and angoline showed good bioavailable response while in network pharmacology analysis, except angoline, all the metabolites significantly interacted with several genes (SOD1, NOS, MAPK3, UGT1As, G6PD, ACOT2, BAAT etc) associated with brain ischemia, oxidative and inflammatory stress or the genes response for elimination of toxins from the body. Conclusion: the study enlightens that A. mexicana possess several major and minor metabolites and which can be the key parameter for further quality and regulatory based assessment of A. Mexicana and biological role against oxidative and inflammatory stress induced epileptic seizure
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