Abstract
Indigofera linnaei Linn..is a potential folklore medicinal plant(Fabaceae) used for Aurveda and Siddha systems of medicine. In this study Alkaloids, Carbohydrate Glycoside, Saponin, Flavonoids, tannins and Phytosteroids were identified as the major phytochemical constituents in the methanol,acetone and toluuenefractions of Indigofera linnaei Linn. leaf extract. Their structures were elucidated, on the basis of GC-MS data.2,4,6-Octanerione (9.24%), 4, (methyl cyclopropyl)-1-butene(8.87%), non-ionic acid methyl ester(5.78%), trans-N-methyl-3-oxo-5,6-dimethoxy morphian(9.63%). (IRS, 2SR) 2-Dimethyl (Phenyl) silylpentane-3-ol(7.39%), 2,2-bis (t-phenyl 3,4” dimethyl phosphate)(5.84%), 2-cyclopropylenetic acid(6.49%)these different active phytochemicals have been found to possess a wide range of activities. In conclusion Indigofera linnaei Linn. contains biologically active compounds that may serve as candidate for the discovery of new drugs in the treatment of antimicrobial activities.
Highlights
Plants have great potential uses, especially as traditional medicine and pharmacopoeial drugs
The filtrate is concentrated by bubbling nitrogen gas into the solution and reduce the volume to 1ml.The extract contains both polar and non-polar phytocomponents. 2.2 GC-MS Analysis: The GC-MS analysis of Indigofera linnaei Linn. powder leaves extract with in absolute alcohol, was performed using a Clarus 500 Perkin Elmer gas chromatography equipped with a Elite-5 capillary column (5% phenyl 95% dimethyl polysiloxane) (30nm X 0.25mm ID X 0.25μmdf) and mass detector turbomass gold of the company which was operated in EI mode
Helium was the carriers gas at a flow rate of 1ml/min. and the injector was operated at 290oC and the oven temperature was programmed as follows; 50oC at 8oC/min to 200oC (5min) at 7oC/min to 290oC(10min). 2.3 Identification of components: Interpretation on mass spectrum of GC-MS was done using the database of National Institute Standard and Technology (NIST), WILEY8, FAME having more than 62,000 patterns
Summary
Plants have great potential uses, especially as traditional medicine and pharmacopoeial drugs. The use of plants with pharmaceutical properties has received increased interest nowadays from bothhomeopathic and allopathic branches These medicinal plants play an important role in public health,especially in developing countries, where it is believed that the intense utilization of plants with therapeutic action does not lead to intoxication 4. The use of plants as therapeutic agents in addition to being used as food is age long.Though the therapeutic uses of plants by the primitive people lack scientific explanations 5, there is agreat awareness in the use and significance of these medicinal floras by the World Health Organization inseveral resource- poor nations 6. This has led to intensified efforts on the documentation of medicinal plants 7
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