Phytochemical and cytotoxic evaluation of Medicago monantha: In vivo protective potential in rats

Abstract

This research focuses on screening and evaluation of bioactive constituents in plants through pharmacological assays. In present study, we evaluated phytochemicals, cytotoxic activity, in vivo effect of M. monantha against CCl4 induced toxicity in cardiac and renal tissues and its aphrodisiac potential in rats. Shade dried plant was extracted with methanol. The phytochemical screening indicates the presence of flavonoids and alkaloids. Aphrodisiac study showed improved sexual desire; may be attributed to the presence of saponins that boosts the androgen level. Cytotoxicity of the plant was assessed through brine shrimp lethality assay and nearly all the fractions showed promising results. The in vivo study focused on the protective ability of extract against CCl4-induced oxidative damage in renal and cardiac tissues of rats. Serum analysis revealed that CCl4 intoxication increased the levels of bilirubin and blood urea nitrogen (BUN). Antioxidant enzyme analysis showed that catalase, peroxidase, superoxide dismutase, glutathione-S-transferase, glutathione activity and protein levels declined due to CCl4 induced renal and cardiac toxicity. Moreover, the histopathological studies of both low & high dose plant treated group’s revealed glomerular hypertrophy and glomerular congestion in kidney, cardiac degeneration and vacuolization of germinal epithelium induced by CCl4 intoxication. DNA also shows damage showed the toxic nature of the plant.