Abstract
Methanolic extract of Curcuma amada leaves was investigated for isolation of secondary metabolites by successive chromatographic separation (VLC, column chromatography and TLC) which yielded several purified compounds. Among them the structures of β-sitosterol and stigmasterol were determined by analysis of high resolution 1H NMR spectroscopic data and co-TLC with authentic samples. The crude methanolic extract of C. amada leaves and its different fractionates i.e. petroleum ether (PESF), chloroform (CSF) and aqueous (AQSF) soluble fractions were evaluated for antibacterial, antioxidant, cytotoxic and thrombolytic activities. In antibacterial screening, the CSF exhibited the highest inhibition against bacterial growth having zone of inhibition 12 mm compared to the standard kanamycin where the zone of inhibition was observed at 18 mm against gram negative Vibrio mimicus. Significant free radical scavenging activity was also exhibited by CSF with the IC50 value of 103.09 μg/ml as compared to tert-butyl-1-hydroxytoluene (BHT) having IC50 31.88 μg/ml. In cytotoxicity study, the crude extract showed significant lethality towards brine shrimp having with the LC50 value of 6.540 μg/ml as compared to the standard vincristine sulfate (0.451μg/ml). In the study for thrombolytic property, different extracts of C. amada exhibited clot lysis ranging from 17.24 to 43.55% as compared to standard streptokinase (93.75 %).
 Dhaka Univ. J. Pharm. Sci. 19(1): 9-13, 2020 (June)
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Dhaka University Journal of Pharmaceutical Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
