Abstract
Abstract The microbiological food qualities of some commercially packed fruit juices sold in India were assessed. A total of 7 health drinks samples were collected including Horlicks, Boost, Tank, Rasna, Rasna plus, TAT Gluco plus and Fruite. Samples were screened for pathogenic bacterial counts which ranged from 1.0x10 1 to 3.0x10 3 cfu/ml. The isolated microbes include vibrio cholera, Salmonella sp, Psedomonas sp, vibrio parahaemolytics, E.coli, Klebseilla sp . The total microbial counts are within acceptable standards for human consumption. vibrio cholera, Salmonella sp, Psedomonas sp, vibrio parahaemolytics, were the most prevalent organisms isolated. All the samples were of acidic pH ranging from 3.20 to 4.50. Titrable acidity was 0.19 to 0.48; sugar (% sucrose) was 0.30 to 13.00. Antimicrobial activity assay of preservative chemicals(Nacl, Sugars, Benzoic acid, Propionic acid, Sodium nitrate) and plant extracts(Tea powder, Aloe vera, Geinger, lemon,) were used against the isolated microbes. Key words: Bacteria, chemical preservatives, plant extracts and antimicrobial activity
Highlights
In today’s world large numbers of medicinal plants and their compound have shown beneficial therapeutic activity (Aqil et al, 2006)
The microbial culture used for the antibacterial test was Bacillus subtilis,Staphylococcus aureus,Escherichia coli,Salmonella typhi,Salmonella paratyphi A,Salmonella paratyphi B,Shigella dysenteriae, Bacillus licheniformis and Pseudomonas aeruginosa which was obtained from the Institute of Environmental studies and Department of Microbiology, University of Karachi
Antibacterial Assay The antibacterial sensitivity of eucalyptus oil was performed by using two methods the disc diffusion method (Anonymous 1993) and by agar well diffusion method (Irobiet et al, 1994)
Summary
In today’s world large numbers of medicinal plants and their compound have shown beneficial therapeutic activity (Aqil et al, 2006). The main phytochemical present in Eucalyptus oil are steroids, alkaloids, tannins, saponins, phenolics, flavonoids, terpenoids glycosides (Saxena et al, 2010, Pathmanathan et al, 2010, Jain et al, 2010). Detection of Tannins Gelatin Test: 1% gelatin solution containing sodium chloride was added in a few drops of oil. Detection of Flavonoids Ferric chloride Test: Ferric chloride solution treated with drops of oil in the test tube that result in the formation of blackish red color point out the presence of flavonoids (Bhandary et al, 2012). Detection of Quinones Few drops of oil were treated with concentrated Hydrochloric acid and observed for the appearance of yellow precipitation (or coloration) (Ugochukwu et al, 2011). Detection of Terpenoids 1 ml of chloroform was treated with 2ml of oil, added a few drops of Concentrated Sulfuric acid.
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