Phytochemical and antibacterial study on native plants of Taleghan

Abstract

Taleghan has a suitable ecological condition for growth of diverse plants. There are many species that are used in folk medicine by inhabitants for the treatment of infection diseases but there further information is required. For this study, approximately 200 species were collected and identified at the Herbarium, school of pharmacy, Tehran University of medical sciences (THE). Pre-Phytochemical tests and antibacterial effect of plant extracts considered. For this proposes hydroalcoholic extracts were prepared using suxhelt apparatus and then dried over vacuum system. Quality tests for consideration of four components: Alkaloid, flavonoid, saponin and tannin, were done via laboratory tests. For antimicrobial effects, total extracts were tested at a concentration of g/ml against eight microorganisms: Streptococcus, Proteus, Escherichia, Salmonella, Staphylococcus, and pseudomonas. Blood agar and Muller Hinton were used as cultures media Result of pre-phytochemical testes as fallows: 84 percent of plant species had Saponin 42 percent of plant species had Tannin 45 percent of plant species had Flavonoid 13 percent of plant species had Alkaloid 69 species had attractive antimicrobial effect and three species including Epilobium hirsutum, Centaurea bruguierana and Centaurea virgata were the best ones. The most sensitive organism was staphylococcus and the most no sensitive was pseudomonas. Phythochemical tests showed that the many of Taleghan’s native species has Saponin and on this result, there is a chance for discovering some suitable species having steroidal components. Numbers of plants with Tannin and Flavonoid were the same and Alkaloid containing plants are very less. Antibacterial tests showed that there is many species effective on tested bacteria’s and the Saponin containing plants are the best ones.