Abstract
Flavonoid extracts from Euphorbia hirta were screened for antibacterial activity by “Disc Diffusion Assay.” Minimum inhibitory concentration (MIC) of extracts was by broth microdilu-tion method. Extracts found sensitive against test pathogens were also evaluated for minimum bactericidal concentration (MBC) and total activity (TA). The highest inhibition zone (IZ) of 17 mm and AI 0.607 ± 0.090 was shown by bound flavonoids of leaves against Agrobacterium tumifacians. Bacillus subtilis was the most sensitive to flavonoids (free and bound) compared to the other bacteria. MIC of the extracts against Bacillus subtilis, Agrobacterium tumefaciens, and Klebsiella pneumoniae was recorded as 0.039 mg.mL−1.
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