Phytochemical and Antibacterial Evaluations of a Medicinal Plant, Carthamus tinctorius L Cultivated in the South West of the Sahara of Algeria in the Wilaya of Adrar

Abstract

This work was carried out on Carthamus tinctorius, on which we performed the extraction of secondary metabolites using cold maceration using a solvent consisting of methanol and acetone and water (7/7/6: V/V/V). We were able to determine the density of polyphenols, flavonoids and condensed tannins present in this medicinal plant. We also studied its antibacterial power tested on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, by a method called “disc diffusion method”. The contents found are 0.25 mg CE/g DM in condensed tannins, 1.17 mg CE/g DM in flavonoids, and 7.18 mg GAE/g DM in polyphenols. The E. coli bacterium is the only one that was sensitive and whose zone of inhibition has a diameter equal to 14 mm. The minimum inhibitory concentration (MIC) of E. coli is equal to 100 mg/mL, and the minimum bactericidal concentration (MBC) is equal to 150 mg/mL; therefore the MBC/MIC ratio of the hydromethanolic extract of C. tinctorius is approximately 1.5, this enabled us to demonstrate that this extract has a bactericidal effect.