Phytochemical analysis of phenolic compounds by using LCMS of Urginea indica

Abstract

Background: Urginea indica is known as a medicinal plant because it contains many chemical compounds with medicinal peroperties. Numerous bioactive components, including alkylresorcinols, bufadienolides, phytosterols, and flavonoids, are present in the plant, particularly in the bulb. The plant possesses antimicrobial, anthelmintic, antidiabetic, anticancer, antioxidant, and wound healing properties, as demonstrated by numerous scientific investigations. This study is carried out to investigate the various chemical constituents that may be present in the plant, including tannins, phenols, alkaloids, flavonoids, glycosides, carbohydrates, resins, quinones, and saponins. Objective: In this study, it was aimed to calculate the total phenolic content from ethanolic extact of bulb of Urginea indica (UI) and to characterize the phytochemicals present in it with the help of LCMS. Materials and Methods: UI collected from Hamirpur himachal Pradesh, and then bulbous parts of UI were air dried. The powdery form of bulb of was extracted with ethanol, and the phytochemical composition of the bulb extracts was analyzed by liquid chromatography with tandem mass spectrometry (LC-MS) method. To determine the total phenolic content the Folin-Ciocalteu method was used. Results: The LC-MS analyses identified 12 compounds in the ethanolic extract of UI. Based on the results it was appeared that UI has phenolic content in its extract.