Abstract
Objective: The research aimed to provide new information regarding the secondary metabolites content of purple and white-purple Orthosiphon aristatus (Blume) Miq. callus, which can then be used as a basis for developing towards cell suspension and ultimately producing secondary metabolites using bioreactors.
 Methods: Callus induction of two varieties of O. aristatus were performed by inoculating sterile leaf explants grown on Murashige and Skoog basal media supplemented with 2,4-dichlorophenoxyacetis acid 0.4 ppm. The secondary metabolites were analysed and quantified using high-performance liquid chromatography with gradient elution.
 Results: The results showed the growth of callus two varieties of O. aristatus in growth media MS with 2,4-D 0.4 ppm. Rosmarinic acid content in the acetone extract of the purple variety callus was 1.28% w/w, and the white-purple variety was 2.22% w/w.
 Conclusion: This study could form the basis for the development of rosmarinic acid production by In vitro culture modification.
Highlights
Orthosiphon aristatus is distributed in China, Africa, Malaysia, India, and Indonesia [1]
Plant determination The results of the two varieties of O. aristatus determination at the School of Life Science and Technology, Bandung Institute of Technology showed that the plant was Orthosiphon aristatus (Blume) Miq. purple flowers and white-purple flowers
Observation of the morphology of the two varieties of O. aristatus showed that the leaves of the two varieties had differences in the leaf veins purple varieties have purple leaf veins and white-purple varieties in green
Summary
Orthosiphon aristatus is distributed in China, Africa, Malaysia, India, and Indonesia [1]. Bogor area is one of the areas where cat whiskers are cultivated in Indonesia [2]. The meaning of Orthosiphon is orthos (straight) and siphon (cylinder). The leaves of O. aristatus have a rhombic shape [4]. Based on the color of flowers and petals, there are O. aristatus purple, white and white-purple varieties. The secondary metabolite content in purple varieties is higher than in white varieties [5]
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