Phytochemical analysis, antioxidant, antibacterial and antidiarrheal activities of Limnophila repens

Afroza Akter,Roni Roy,Sharmin Sultana,Mohammad Anwarul Basher,A F M Shahid Ud Daula

doi:10.14499/indonesianjpharm30iss3pp187

Afroza Akter, Roni Roy + Show 3 more

Open Access

https://doi.org/10.14499/indonesianjpharm30iss3pp187

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Abstract

The study was carried out to determine the phytochemical content, antioxidant, antibacterial and antidiarrheal activity of methanolic extract of whole plant of Limnophila repens . The total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found to be 65.21 ± 0.004 mg GAE/g , 44.83 ± 0.003 mg QE/g , 17.21 ± 0.002 mg QE/g, 56.27 ± 0.002 mg GAE/g , 1.32 ± 0.01 µg/g, 0.93 ± 0.012 µg/g, 11.85 ± 0.04 mg/g and 9.69 ± 0.037 mg/g, respectively. In vitro antioxidant potential of L. repens was assessed using various methods like by 2,2-diphenyl-1 picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, reducing power assay, FRAP assay and thiobarbituric acid assay. IC 50 values of the aforesaid tests were found to be 2.33 mg/ml, 442.31 µg/ml, 374 µg/ml, 55.09 µg/ml, and 4.30 mg/ml, respectively. The total antioxidant capacity was observed 140.7 ± 0.004 mg/g AAE. Aantibacterial activity of the methanolic extracts of whole plant of L. repens was evaluated using the disk diffusion method. The plant extract didn’t show any activity against Gram-positive (except Staphylococcus aureus ) and Gram-negative bacteria. Finally, castor oil induced method was conducted to investigate the antidiarrheal activity of L. repens . At the dose of 400 mg/kg, the plant extract (35.62% inhibition of diarrhea) exhibited stronger effect compared to standard drug, loperamide (27.4% inhibition of diarrhea).

Highlights

Limnophila repens, commonly known as ‘creeping meshweed’ is a herb widely distributed in South and South-East Asia, China, Pacific islands and Australia (Rahman, 2006)
Despite its use as folk medicine and other putative therapeutic potentials, no quantitative phytochemical analysis and examination of therapeutic effects of L. repens have been performed against important health concerns of Bangladesh– in diarrhoeal disease
The majority of biological active constituents were present in methanol extracts of L. repens in considerable amount

Summary

INTRODUCTION

Limnophila repens, commonly known as ‘creeping meshweed’ is a herb widely distributed in South and South-East Asia, China, Pacific islands and Australia (Rahman, 2006). The herb Limnophila repens mainly grows in marshy places in low lying areas. It is traditionally used in the treatment of leukoderma. Despite its use as folk medicine and other putative therapeutic potentials, no quantitative phytochemical analysis and examination of therapeutic effects of L. repens have been performed against important health concerns of Bangladesh– in diarrhoeal disease. To confront this disease, in this present study, we performed experiments on phytochemical analysis, in vitro antioxidant activity, and in vivo antidiarrhoeal activity of methanol extract of whole plant of L. repens

MATERIALS AND METHODS

AND DISCUSSION

Findings

CONCLUSION