Abstract
Indian Journal of Natural Products,2021,35,1,32-37.DOI:10.5530/ijnp.2021.1.7Published:February 2021Type:Original ArticleAuthors:Renuka Mahajan, and Prakash Itankar Author(s) affiliations:Renuka Mahajan, Prakash Itankar Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, INDIA. Abstract:Aim: The study aims screening of phytochemicals and standardization of extract of Pedalium murex Linn. fruits. Methods: The extraction was carried out by maceration technique using 70% ethanol and 30% water. The aqueous ethanolic extract obtained was estimated for total phenolic and flavonoid content against the standard gallic acid and quercetin. Liquid –liquid partitioning method using solvents n-hexane, chloroform, ethylacetate provided the different fractions of aqueous ethanolic extract. Standardization was done with highly sensitive, accurate, easy and cost effective high performance liquid chromatographic method. Results: The preliminary phytochemical screening of aqueous ethanolic extract of P. murex fruits revealed the presence of alkaloids, saponins, tannins, flavonoids, sugar, glycosides, phenols and sterols. The total phenolic content determined using linear regression equation (y = 0.0123x - 0.0096 R² = 0.9962) was found to be 27.1± 0.72 mg/g equivalent of gallic acid while the flavonoid content was expressed as 17.6 ± 0.79 mg/g equivalent of quercetin. The mother extract and the aqueous fraction revealed peaks complementary with the chromatogram of standard quercetin addressing presence of quercetin. Conclusion: The study helped in developing the phytochemical profile of the extract and the standardization performed in the study confirmed the presence of quercetin in the mother extract and fractions. Keywords:Flavonoid content, HPLC, Liquid- liquid partitioning, Maceration, Phenolic content, Phytochemical screeningView:PDF (589.21 KB)
Highlights
Various health ailments have been traditionally treated using medicinal plants since ancient times
The percent yield is the ratio of the weight in grams of extract to the weight of powdered drug taken, multiplied by 100.[13]. The preliminary phytochemical screening of aqueous ethanolic extract of P. murex fruits revealed the presence of alkaloids, saponins, tannins, flavonoids, sugar, glycosides, phenols and sterols[14] Table 2
0.0123x - 0.0096 Correlation coefficient (R2) = 0.9962, R2 represents correlation coefficient) was found to be 27.1± 0.72 mg/g equivalent of gallic acid while the flavonoid content was expressed as 17.6 ± 0.79 mg/g equivalent of quercetin Table 3.[15]. Standardization of herbal drugs is a matter of interest and concern for herbal drug industry.[16]
Summary
Various health ailments have been traditionally treated using medicinal plants since ancient times. Plants are the reservoir of naturally occurring bioactive compounds for drug discovery. These compounds serve as remedy for variety of diseases and are of extreme interest in modern therapeutics.[1] Pedalium murex L. commonly designated worldwide as large caltrops has been consistently evaluated, past five decades, for its biological activity and medicinal properties. The plant belonging to the family Pedaliaceae is called as Gokshura, Gokantaka, Bara gokhru, Khaar-e-kkhasak, Peru neranji and with few more names in other languages. It is commonly found throughout India especially in Southern coastal region i.e. Kokan, Gujrat, Kathiawar, Ceylon, Peninsula and Deccan. The plant being loch of naturally occurring bioactive metabolites, the work was undertaken to analyze the phytochemical profile and develop fingerprinting of hydro alcoholic extract and fractions for standardization of extract
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
