Phytochemical Analysis and Assessment of In vitro Antioxidant Potential of Polyherbal Formulation

Abstract

Background:Herbal remedies exhibit a wide variety of bioactive components, creating them an extensive source of numerous types of treatments. Due to various safety issues, natural antioxidants are now favoured over synthetic antioxidants. There is growing demand for natural antioxidants such as polyphenols, which have been reported in herbal plants and may help to prevent oxidative stress and inflammation. Natural antioxidants boost plasma antioxidant potential and minimize the risk of illness. Aims and objective: The current study is concerned with the phytochemical analysis of polyherbal emulgel and the assessment of in vitro antioxidant activity. Method: Polyherbal emulgel was analyzed for various phytochemical constituents and antioxidant activity using DPPH method. Results: Based on the various phytochemical tests, N. jatamansi indicated the presence of Alkaloids, Carbohydrates, Flavonoids, Proteins and Saponins. A. paniculata exhibited the existence of Alkaloids, Flavonoids, Tannins, steroids and Saponins. While, C. paniculatus displayed the occurrence of Alkaloids, Flavonoids, Tannins and Steroids. F-2 (N. jatamansi) formulation showed more potent antioxidant activity with %Inhibition of 56.35±1.58 at 150µg/ml than standard (BHT) with %Inhibition of 40.60±1.49 at 150µg/ml, while F-6 (A. paniculata and C. paniculatus), F-4 (N. jatamansi and A. paniculata) and F-7 (N. jatamansi, A. paniculata and C. paniculatus) produced comparable antioxidant activity to that of standard drug BHT. Conclusion: Emulgel F-2 (N. jatamansi), F-4 (N. jatamansi and A. paniculata), F-6 (A. paniculata and C. paniculatus) and F-7 (N. jatamansi, A. paniculata and C. paniculatus) possess potent antioxidant activity which requires further in vivo studies.