Phytochemical analysis and antioxidant activity of honeys from wild colony and bee culture from five regions of the ivory coast

Abstract

Honeys have a very complex biological activity due to their composition of phenolic compounds which are very useful for the treatment of certain conditions. The objective of this study is to analyze the phenolic compounds and antioxidant activity of honeys from wild colonies and beekeeping on the Ivory Coast. This study was carried out in five regions of Côte d’Ivoire (Poro, Tchologo, Hambol, Bélier and N’zi). Twenty (20) samples from honey from wild colonies and beekeeping were collected and analyzed. The polyphenol content was quantified by the Folin-Ciocalteu colorimetric method. The aluminum trichloride (AlCl3) method was used to quantify the flavonoids in the extracts. The quantitative evaluation of the antioxidant potential of honeys was carried out by the DPPH test. The tests revealed the presence of polyphenols, tannins and flavonoids. High polyphenol contents were obtained with MSP (4.32 ± 0.02 mgEAG/g), MSH (3.7 ± 0.01 mg EAG/g), MAIPH (3.16 ± 0.02 mgEAG/g) honeys. g), MST (2.95 ± 0.01 mgEAG/g) and MSB (2.74± 0.02 mgEAG/g). MSH honey samples (1.48 ±0.05 mg QE/100 g); MSP (1.36 ± 0.03 mg QE/100 g) recorded the highest flavonoid values. The MAPIH sample (2.56 ± 0.04 mg EQ/g) recorded the highest tannin value. Significant antioxidant capacities were observed in honeys from wild Poro MSP colonies (82.384% at 80 mg/ml) IC50= (44.86 mg/ml).