Abstract
Achillea millefolium L. commonly known as yarrow belongs to family Asteraceae. Phytochemical analysis of plant A. millefolium has been done by phytochemical screening, fourier transform infrared spectroscopy and gas chromatography- mass spectrometry. Antibacterial activity of plant A. millefolium has been done by disc-diffusion method against E. coli, S. aureus, B. subtilis and P. aeruginosa. Phytochemical screening confirmed the presence of alkaloids, cardiac glycosides, flavanoids, phenolic compounds, tannin, terpenoids and phlobatannin. Maximum antibacterial activity was shown by vegetative A. millefolium (V) with MIC 50 μg/ml, maximum ZOI against B. subtilis of 19±0.5 mm and maximum % increase in ZOI against B. subtilis i.e. 80% and 40.74% as compared to standard tetracycline and streptomycin respectively. FT- IR and GC- MS of A. millefolium revealed presence of different functional groups and active compounds respectively responsible for antibacterial activity.
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