Abstract
Use of nanomaterials as antimicrobial agents is gaining tremendous importance. In the present investigation, bimetallic nanoparticles were synthesized using an aqueous extract of Annona squamosa L. Initial confirmation was attenuated with a change in color of the reaction mixture and further confirmation was achieved with UV–Visible spectrophotometry which displayed absorbance peak between 500 and 700 nm. The possible role of phyto-components present in aqueous extract to mediate and stabilize nanoparticles was studied using Fourier transform infrared (FTIR) analysis which predicted the presence of hydroxyl, carboxyl and amide functional groups. The X-ray diffraction (XRD) analysis revealed the crystalline nature of bimetallic nanoparticles. The morphological characteristics of bimetallic nanoparticles were studied using Transmission electron microscopy (TEM) which depicted the polydispersity of nanoparticles with myriad size and shapes. The antibacterial activity was assessed with well diffusion, minimal inhibitory concentration. The bimetallic nanoparticles were effective against tested human pathogens and Bacillus subtilis (MTCC 121) followed by Staphylococcus aureus (MTCC 7443), Escherichia coli (MTCC 7410) and Salmonella typhi (MTCC 7407). The obtained preliminary investigation is interesting for future studies to elucidate the mechanism of synthesis and the possible mode of action of bimetallic nanoparticles against the test pathogens.
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