Phyto-stimulating activity of the paracetamol-containing waste biodestruction product

Abstract

Currently, the search for effective ways to dispose of pharmaceutical waste, including the use of microgerms, is relevant, which makes it possible to obtain products with new useful, in particular, phytostimulating properties. Our previous researches have shown that the product of bacterial destruction of paracetamol exhibits frank phytostimulating properties toward medicinal plants of the Lamiaceae, Plantaginaceae, Urticaceae, Asteraceae can be used as an inducer of accumulation of biologically active substances in them.
 The purpose of this work is to investigate the effect of the biodegradation product of paracetamol on the amount of glycans, biomass, size and anatomical features of linseeds Linum usitatissimum L. (Linaceae family).
 Materials and methods. The work used a product of biodegradation of paracetamol (PBP) obtained on the basis of the laboratory of alkanotrophic microorganisms of the Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences (Perm) from the pharmaceutical substance paracetamol with an expired expiration date. The study of the phytostimulating effect of PBP in relation to common flax was carried out on the basis of the A. G. Genkel Botanical Garden of the Perm State National Research University. The content of glycans was determined by gravimetric method. A comparative analysis of the anatomical structure of seeds was carried out using the Motic DM-111 digital microscope and the Motic Play and Motic Educator software.
 Results. When processing flax seedlings of ordinary PBP, the amount of glycans in seeds increased by 6%, the total collection of seed biomass by 20.5%, the size (length) of seeds by 2% compared to the control (water). Biometric parameters of the mucous layer of seeds collected from the area treated with PBP increased by 10% the control in comparison.
 Conclusion. The biodegradation product of paracetamol has a stimulating effect on the medicinal plant of the flax family (Linaceae) flax Linum usitatissimum L., increasing the content of polysaccharides, the total collection of seed biomass and their size.