PHYTO-CONSTITUENTS OF CROTON BONPLANDIANUS AS ECO-FRIENDLY BIO-WEAPON AGAINST HUMAN VECTOR MOSQUITOES

Abstract

The Croton bonplandianus various leaf extracts were assessed the presence of phytochemical in which predominant numbers occupied by high polarity solvent (methanol extract). ‘+’ denoted as presence of phytochemical group and ‘-’ denoted as absence of phytochemical group. The major phyto-compounds of Dihydro-pseudosolasodine and Methylsulfonic acid, 2,2,2-trichloroethyl ester were identified from C. bonplandianus leaf methanolic extract by using GC-MS analysis. The major phyto-constituents were tested by standard protocol with various concentrations (4-250μg/mL) against 3rd instars larvae of different vector mosquitoes Ae. aegypti and Cx. quinquefasciatus By GC-MS analysis, confirmed the presence of 15 phyto-compounds in which, Dihydro-pseudosolasodine (15.03%) and Methylsulfonic acid, 2,2,2-trichloroethyl ester (17.72%) were noticed as major phyto-constituent. The lethal toxicity (LC50/LC90) of C. bonplandianus leaf methanol extract and Dihydro-pseudosolasodine and Methylsulfonic acid, 2,2,2-trichloroethyl ester tested against 3rd instar larvae of Ae. aegypti and Cx. quinquefasciatus values were 78.48/178.68, 80.33/180.32, 11.46/19.90, 11.72/19.66, 10.66/19.06 and 10.71/19.78 µg/mL, respectively. C. bonplandianus leaf methanol extract and selected phyto-compounds were exposed with juvenile stage of medical vector which found the hyper toxicity at lower concentration. Our results, the C. bonplandianus phyto-pesticides achieved many folds topper toxic effects on medical vectors which provided eco-friendly approaches to environment.