Abstract
Isolation and enumeration of autochthonous heterotrophic gut microbiota from four carps (Labeo rohita, Catla catla, Hypophthalmichthys molitrix and Labeo bata) has been carried out. Gut isolates were analyzed qualitatively for extracellular amylase, protease, cellulase and lipase producing capacities and four superior isolates were further studied for phytase producing capabilities. The isolate CC1.1 (Rhodococcus sp. MTCC 9508) isolated from C. catla seemed to be the best phytase producer amongst the isolates studied. Bio-safety of the isolate was evaluated in vivo by injecting fresh broth culture to healthy laboratory acclimatized rohu, L. rohita. Presence of autochthonous phytase producing Rhodococcus sp. detected in the present study offers scope for further research to appraise its potential application in commercial aquaculture.
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