Physiology of acclimation to salinity stress in pea (Pisum sativum)

Abstract

Pea (Pisum sativum L.) seedlings were grown in half strength Hoagland solution and exposed to 0, 10, 25mM NaCl and 2.5% PEG 6000 for 1 week (pre-treatment). Thereafter plants were exposed to 0 and 80mM NaCl for 2 weeks (main treatment). The control plants were maintained in half strength Hoagland solution without NaCl. Various physiological parameters were recorded from control, pretreated and non-pretreated plants. There was no negative effect of the pre-treatments on growth (total fresh and dry matter production), and plants pre-treated with 10mM NaCl had biomass accumulation equal to control plants. The beneficial effect of salt acclimation was also evident in the prevention of K+ leakage and Na+ accumulation, primary in roots, suggesting that here the physiological processes play the major role. 2.5% PEG 6000 was not as efficient as salt in enhancing salt tolerance and acclimation appears to be more related to ion-specific rather than osmotic component of stress. We also recorded an increase of the xylem K/Na in the salt acclimated plants. Therefore, the present study reveals that short-term exposure of the glycophyte P. sativum species activates a set of physiological adjustments enabling the plants to withstand severe saline conditions, and while acclimation takes place primary in the root tissues, control of xylem ion loading and efficient Na+ sequestration in mesophyll cells are also important components of this process.