Physiological Responses of Two Varieties of Common Bean (Phaseolus VulgarisL.) to Foliar Application of Silver Nanoparticles

Ahmed Ibrahim El-Batal,Amal Zakaria Hegazi,Fatma Abd El-Lateef Gharib,Asmaa Gamal Mohamed Abd El Hafz,Safia Mohammed Ghazi

doi:10.5772/62202

Abstract

This study reported the synthesis of aqueous dispersions of highly stable silver nanoparticles (AgNPs) using gamma radiation with gum acacia (GA) as the stabilizing and protecting agent. The formation of nanosized silver GA-AgNPs at a gamma irradiation dose of 15 kGy was confirmed by the appearance of the characteristic surface plasmon absorption peak at 456 nm and in UV–vis spectra. The particle size distribution of silver nanoparticles that was determined by Dynamic Light Scattering (DLS) was approximately 22 nm. By using Transmission Electron Microscopy (TEM), mostly spherical GA-AgNPs particles with a mean diameter of 16.7 nm and crystalline in shape were confirmed by an X-ray diffraction (XRD) pattern. Fourier Transform Infrared (FTIR) spectroscopic analysis indicates the bonding of AgNPs with the COO- group of gum acacia. To increase our understanding of the mechanisms involved in plant responses to GA-AgNPs and AgNO3, in order to differentiate between particle-specific and ionic-silver effects, we ...

Highlights

Nanotechnology has become a growing concern in recent years, with the unlimited production of nanoparticles (NPs) and their successful applications in broad fields
Different doses of gamma irradiation have the same effect on the plasmon resonance on the peak position at 5 kGy and 30 kGy gamma irradiation; a peak appeared at the SPR of silver nanoparticles at about 456 nm
The present study demonstrates the biological mechanism for biosynthesis and the rapid production of high yield, stable gum arabic-capped silver nanoparticles (GAAgNPs) with a mean diameter of 16.7 nm using 1 mM AgNO3 and gamma irradiation at 15 kGy doses

Summary

Introduction

Nanotechnology has become a growing concern in recent years, with the unlimited production of nanoparticles (NPs) and their successful applications in broad fields. Green synthesis is an alternative harmless and environ‐ mentally friendly method for producing nanoparticles [17] Natural polymers such as sodium alginate [18], carboxy‐ methyl cellulose sodium [19], heparin and extract of Memecylon umbellatum leaf have been used for the capping of silver and gold nanoparticles [20, 21]. Gamma irradiation has been proven to be a simple and efficient method for silver nanoparticles’ synthesis. It requires an aqueous system, room temperature and ambient pressure [10,11,12,13, 23]. Shifts in intensity and wavelength of the SPA absorption spectrum followed the growth in particle size [24]

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Conclusion