Physiological responses of the green alga Dunaliella parva (Volvocales, Chlorophyta) to controlled incremental changes in the N source.

Abstract

This work is aimed at obtaining information on the acclimation processes of the green flagellate Dunaliella parva Lerche to gradual changes in the N source from NO3- to NH4+, in continuous cultures. Photosynthesis, dark respiration, and light-independent carbon fixation (LICF) rates, chlorophyll a fluorescence, RUBISCO and phosphoenolpyruvate carboxylase (PEPc) activities, plasmalemma electrical potential difference, cell volume, and absolute or relative amounts of major cell constituents were measured. Two phases characterised the response to the transition from NO3- to NH4+: (1) an initial phase in which photosynthesis and anaplerosis were stimulated and protein increased; (2) a subsequent phase in which most parameters reached new values that were close to those at the beginning of the experiment (100% NO3-). The only exceptions were PEPc activity and LICF, whose rates remained at least 2-fold higher than at 100% NO3-, when NH4+ was the sole N source. The results are indicative of a tendency to re-establish homeostasis, after an initial perturbation of the intracellular composition. The roles of different metabolic processes during acclimation are discussed.