Physiological responses of macroalga Gracilaria lemaneiformis (Rhodophyta) to UV-B radiation exposure

Abstract

This paper aims to evaluate the effects of ultraviolet-B radiation (UVBR) on Gracilaria lemaneiformis, a commercial red macroalga and an important source of agar. To study the in-vitro effect of UVBR on G. lemaneiformis, this plant was cultivated and exposed to photosynthetically active radiation (PAR) at 40 μmol photons/(m2 ·s) and enhanced UVBR (0, 0.36, 0.72, 1.08, 1.44, and 1.80 kJ/(m2 ·d)) for 13 days. The samples were processed for histochemical analysis, and the growth rate, photosynthetic pigment contents, photosynthetic performance, reactive oxygen species levels, membrane permeability, malonyl dialdehyde contents and antioxidant capacity of G. lemaneiformis were investigated. After 13 days of exposure to PAR+UVBR, G. lemaneiformis showed photodamage and photoinhibition of photosynthetic pigments (chlorophyll a and phycoerythrin), leading to a decreased photosynthetic efficiency. Further, there was a corresponding decrease in the relative growth rates and depigmentation and partial necrosis of the apical segments were noted after exposure to PAR+UVBR. Additionally, UVBR induced excess production of superoxide radicals and hydrogen peroxide, eliciting a marked cellular membrane damage and antioxidative response.