Physiological Response of Vitis Amurensis Rupr. Seedlings to Drought Stress and Re-watering

Abstract

In order to study drought resistance in Vitis amurensis Rupr. seedling and to provide some theoretical bases for water management in Vitis amurensis Rupr. production, using potted ‘Beta’ tissue culture seedlings as materials, effects of natural drought stress for different durations and re-watering treatment on physiological indexes of leaves. The results showed that drought stress can cause cells dehydration of Vitis amurensis Rupr., and this reaction intensifies with time. Especially after 20 d drought stress re-watering, the relative water content of leaves has no obvious change compared with drought treatment, that is, it has caused loss to cell membrane, while the effect of short- and medium-term drought (5 d, 10 d) is relatively small. The content of proline and soluble sugar increased first and then decreased with the extension of drought stress time, and the osmotic regulator increased relatively steadily in the short time (5 d, 10 d), which indicated that it could maintain metabolic balance through its own osmotic regulation. The accumulation of proline and soluble sugar decreased under prolonged stress (20 d), the accumulation of proline and soluble sugar was significantly higher than the control, which still had some protective effect on the cells. Under short drought stress, the activity SOD, POD, CAT grape increased steadily, but with the prolongation of stress time, the activity of the three protective enzymes varied greatly. The SOD activity reached a peak d stress 15, and the POD, CAT activity reached a peak in 20. After re-watering, the three protective enzyme activities of Vitis amurensis Rupr. decreased, and after 10 d of stress, the protective enzyme activity of re-watering could be restored to the control level. It shows that the grape has a certain ability to recover after drought. To sum up, this study suggests that Vitis amurensis Rupr. seedlings can withstand 10 d of drought stress because of their high osmotic regulation and antioxidant capacity to maintain normal physiological metabolism.