Abstract
Grapevine leafroll disease is one of the most severe viral diseases of grapevine caused by Grapevine leafroll-associated viruses (GLRaVs). Physiological processes were monitored on grapevines with single (GLRaV-1) and mixed (GLRaV-1 and GLRaV -3) viral infection under greenhouse conditions from June to September, in vegetation period 2014. In the mid of the season (July) negative effects of the virus infections on physiological processes were more severe in mixed than in single infection. The net-photosynthesis (Pn) of the leaves infected with GLRaV-1 and GLRaV-3 reached only a half of the Pn in GLRaV-1 infected grapevines. Similar reduction was found for stomatal conductance, transpiration and parameters related to photochemical efficiency (electron transport rate).
Highlights
Grapevine leafroll disease is one of the most severe viral diseases of grapevine caused by Grapevine leafroll-associated viruses (GLRaVs)
Razumevanje vpliva GLRaV na vinsko trto otežuje kompleksnost virusov in zelo raznolik fiziološki odziv rastlin na virusne okužbe (Balachandran in sod., 1997)
Nasprotno so Tomažič in sod. (2005) ugotovili, da je bila kakovost grozdja pri trtah okuženih z GLRaV-1 boljša kot pri trtah brez tega virusa, verjetno tudi zaradi manjšega pridelka in posledično hitrejšega dozorevanja jagod okužene trte (Tomažič in sod., 2005)
Summary
V novembru 2013 smo po predhodnem testiranju s DAS-ELISA testom na GLRaV-1 in GLRaV-3 narezali rozge žlahtne vinske trte ('Blaufränkisch’('Modra Frankinja'), 'Rheinriesling' ('Renski rizling') in 'Pinot Gris' ('Sivi Pinot') okužene z GLRaV-1 ter z GLRaV-1 in GLRaV-3. Trte smo zalivali vsak četrti dan oziroma po potrebi in tudi 24 ur pred vsakimi fiziološkimi meritvami. V času poskusa smo trte tretirali s sistemičnima pripravkoma z aktivno snovjo heksitiazoks (akaricid) in tebukonazol (fungicid) ter pognojili z raztopino gnojila NPK 6-3-6 z mikrohranili in dodatkom morskih alg (Unichem). Odvzem vzorca smo opredelili kot sistematično napako (enaka poškodba na merjenih listih pri vseh trtah v poskusu), ki je imela zanemarljiv vpliv na meritve, saj poteka odziv na ranitveni stres navadno le nekaj ur (León in sod., 2001). Opazovali smo tudi pojavljanje bolezenskih znamenj na preučevanih trtah
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