Physiological integration for salinity stress alleviation in stoloniferous turfgrass, Zoysia matrella in heterogeneous saline environments

Abstract

To clarify physiological integration for salinity stress alleviation in Zoysia matrella, interconnected clonal stolon ramets were grown in three separate pots [basal (BR), intermediate (IR), and apical ramets (AR)]. Once ramets were well established, two experimental groups were set up: stolons connected with other ramets (Intact) and stolons cut between ramets (Severed). NaCl solution was used for IR, while tap water was used for BR and AR for 49 days. For BR and AR, there was no significant difference in NaCl content in any plant part (green leaves, brown leaves, stolons, and roots) in both groups. Therefore, we concluded that no NaCl translocation in interconnected ramets occurred for both basal and apical directions. For the IR in Intact group, water content in stolons was 1.2 times higher, and NaCl content in green and brown leaves was 16 and 29% lower, respectively than in Severed group. Dry weight of both above ground and below ground parts in Intact groups were 1.3 times higher as compared to that in Severed group. It was considered that tap water treated ramets (BR or AR) translocated water to IR and decreased the amount of NaCl in the body, thereby alleviating salt stress. For the IR in both groups, NaCl content in brown leaves was 2–3 times higher than in other parts. We conclude that Zoysia matrella does not translocate toxic component, such as NaCl between ramets, and does not distribute it to other ramets; however, ramet accumulates NaCl in brown leaves under salt stress condition.