Abstract
Stability of erythrocyte-reduced glutathione in the presence of acetylphenyl-hydrazine (“glutathione-stability test”) was studied in healthy and riboflavin-deficient subjects. Many apparently healthy subjects showed poor stability, which improved after treatment with riboflavin. However, clinically riboflavin-deficient subjects with very low glutathione reductase activity, had satisfactory glutathione stability, which tended to show a slight fall after treatment. These results indicate the presence of a compensatory mechanism which helps the cell to overcome glutathione reductase deficiency. Riboflavin-deficient subjects had normal glucose-6-phosphate dehydrogenase levels and normal K m of glutathione reductase for NADPH. Destruction of glutathione by acetylphenylhydrazine occurs even in boiled erythrocyte hemolysate.
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